Use of Randomly
Amplified Polymorphic DNA (RAPD) analysis as a tool for determining the genetic diversity
within populations of the invasive Asiatic sand sedge, Carex kobomugi.
Hendry, K. (student), Vestyck, L., Richtmeyer, M. and Wootton,
L. Georgian Court University, 900 Lakewood Ave,
Lakewood NJ 08701.
Approximately 100 million acres of land in the United States have
been invaded by non-native plants in the past century. The Asiatic sand sedge, Carex kobomugi is one such invasive plant. It displaces more fragile native species and seems
to be without natural predators. How this species reached the U.S. is unknown, although it
may have been used as packaging material for imported china, and a wrecked ship carrying
such cargo may be responsible for its introduction into our area. Since then, it has
expanded rapidly along the Jersey coast in habitats similar to American Beach Grass,
reducing native species richness and diversity. C.
kobomugi reproduces both sexually and asexually. Understanding
the relative importance of each of these in the species dispersal may provide
important insights into how it may best be controlled. To determine this, we are using
Randomly Amplified Polymorphic DNA analysis of samples collected in New Jersey, as well as
samples obtained from Japan, to assess the genetic diversity within the species. We expect
that, if reproduction is primarily asexual, the DNA will show that the NJ plants are
clones of one another, whereas if the plants use sexual means then they will be
genetically diverse.